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mouse anti sam68 primary antibody  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse anti sam68 primary antibody
    Mouse Anti Sam68 Primary Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 249 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+sam68/Sam+68/pm41725810-106-30-34
    Average 92 stars, based on 249 article reviews
    mouse anti sam68 primary antibody - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    other:

    Article Title: The effect of inhibition of receptor tyrosine kinase AXL on DNA damage response in ovarian cancer.
    Article Snippet: Primary antibodies used were anti-AXL (CS8661; C44E1), anti-ATM (92356 S), anti-pATM (13050S), anti-ATR (13934 S), anti-pATR (58014S), antiCHK1 (2360S), anti-pCHK1 (2348S), anti-CHK2 (6334S) and anti-pCHK2 (2197S) from Cell signalling; anti-SAM68 (07-415) and anti-γH2AX (3BW301) from Millipore; antiRPA2 [p Ser33] (NB100-544) from Novus Biologicals; anti-SAM68 (ab76471), anti-SREBP2 (30682), anti-HMGCR (242315) from Abcam; antisqualene synthase (sc-271602), anti-MVK (sc-390669), anti-OSC (sc-514507), antipADPr (sc-56198), anti-GAPDH (sc-47724), anti-ß-actin (sc-47778) from Santa Cruz.

    Incubation:

    Article Title: Comparative O -GlcNAc Proteomic Analysis Reveals a Role of O -GlcNAcylated SAM68 in Lung Cancer Aggressiveness.
    Article Snippet: .. After PBS washing, fixed cells were incubated with primary antibodies, including anti-OGT (1:200; 11576-2-AP; ProteinTech), anti-SAM68 (1:200; sc-333; Santa Cruz Biotechnology Inc.), anti-Myc (1:100; MMS-150P; Covance, Richmond, CA, USA) or anti-Flag (20 μg/mL; F3165; Sigma-Aldrich), at 4 ◦C overnight. ..

    Article Title: Comparative O -GlcNAc Proteomic Analysis Reveals a Role of O -GlcNAcylated SAM68 in Lung Cancer Aggressiveness
    Article Snippet: .. After PBS washing, fixed cells were incubated with primary antibodies, including anti-OGT (1:200; 11576-2-AP; ProteinTech), anti-SAM68 (1:200; sc-333; Santa Cruz Biotechnology Inc.), anti-Myc (1:100; MMS-150P; Covance, Richmond, CA, USA) or anti-Flag (20 μg/mL; F3165; Sigma-Aldrich), at 4 °C overnight. ..

    Article Title: Human papillomavirus 16 E6 oncoprotein promotes up-regulation of RNA-binding protein Sam68 in head and neck cancer.
    Article Snippet: .. Cells were fixed in 4% paraformaldehyde and permeabilized for 10 min in 1X PBS containing 0.1% TritonX-100, and incubated overnight with anti-Sam68 (Santa Cruz Biotechnology, RRID: not available, sc-514468, 1:100 dilution) or anti-HPV16 E6/18 E6 (C1P5) (Santa Cruz Biotechnology, RRID:AB_675656, sc-460, 1:50 dilution) antibodies in blocking solution (1X PBS, 0.05% TritonX-100, 5% FBS). .. The Anti-phalloidin Alexa Fluor 647 (Invitrogen, A22287), and 4 μM Hoechst 33342 (Thermo Fisher Scientific) were used for cytoskeleton and nuclei counterstaining, respectively.

    Article Title: Substrate-bound and soluble domains of tenascin-C regulate differentiation, proliferation and migration of neural stem and progenitor cells
    Article Snippet: Cells were detached from the dish using a cell scraper (Sarstedt, Nümbrecht, Germany, Cat# 83.1832). .. For SDS-PAGE, 12 μl protein sample was mixed with 3 μl fourfold sample buffer and heated at 95°C for 5 min. SDS-PAGE and western blot was performed as described in section “2.2 Detection and verification of the Fc recombinants.” For protein detection the membranes were incubated at 4°C over night on a rocker with following primary antibodies: anti-phospho extracellular signal-regulated kinases (pErk; 1:1000; rabbit; Cell Signaling Technology; Cat# 9101; RRID:AB_331646 ), anti-total Erk (tErk; 1:1000; mouse; Santa Cruz Biotechnology; Cat# sc-271269; RRID:AB_10611091 ), anti-phospho Akt (pAkt; 1:2000; rabbit; IgG; Cell Signaling Technology; Cat# 4060; RRID:AB_2315049 ), anti-total Akt (tAkt; 1:1000; rabbit; IgG; Cell Signaling Technology; Cat# 4691; RRID:AB_915783 ), anti-phospho focal adhesion kinase (pFAK; 1:1000; rabbit; Cell Signaling Technology; Cat# 3283; RRID:AB_2173659 ), anti-total FAK (tFAK; 1:1000; rabbit; Cell Signaling Technology; Cat# 3285; RRID:AB_2269034 ), anti-notch (1:1000; rabbit; IgG; Cell Signaling Technology; Cat# 3608; RRID:AB_2153354 ), anti-cleaved notch (1:1000; rabbit; IgG; Cell Signaling Technology; Cat# 4147; RRID:AB_2153348 ), anti-Sam68 (Src associated in mitosis, of 68 kDa; 1:500; mouse; IgG; Santa Cruz Biotechnology; Cat# sc-1238; RRID:AB_627858 ) and anti-α-tubulin (1:10000; mouse; IgG; Sigma-Aldrich; Cat# T9026; RRID:AB_477593 ; see ) diluted in 5% (w/v) milk powder in 1xTBST. .. The membranes were washed thrice with 1x TBST and incubated at room temperature for 1 h on a rocker with following HRP-coupled secondary antibodies: anti-mouse [1:5000 (1:10000 for α-tubulin); goat; IgG; Jackson ImmunoResearch Labs; Cat# 115-035-068; RRID:AB_2338505 ] and anti-rabbit (1:5000; goat; IgG; Jackson ImmunoResearch Labs; Cat# 111-035-144; RRID:AB_2307391 ; see ) diluted in 5% (w/v) milk powder in 1xTBST.

    Blocking Assay:

    Article Title: Human papillomavirus 16 E6 oncoprotein promotes up-regulation of RNA-binding protein Sam68 in head and neck cancer.
    Article Snippet: .. Cells were fixed in 4% paraformaldehyde and permeabilized for 10 min in 1X PBS containing 0.1% TritonX-100, and incubated overnight with anti-Sam68 (Santa Cruz Biotechnology, RRID: not available, sc-514468, 1:100 dilution) or anti-HPV16 E6/18 E6 (C1P5) (Santa Cruz Biotechnology, RRID:AB_675656, sc-460, 1:50 dilution) antibodies in blocking solution (1X PBS, 0.05% TritonX-100, 5% FBS). .. The Anti-phalloidin Alexa Fluor 647 (Invitrogen, A22287), and 4 μM Hoechst 33342 (Thermo Fisher Scientific) were used for cytoskeleton and nuclei counterstaining, respectively.

    SDS Page:

    Article Title: Substrate-bound and soluble domains of tenascin-C regulate differentiation, proliferation and migration of neural stem and progenitor cells
    Article Snippet: Cells were detached from the dish using a cell scraper (Sarstedt, Nümbrecht, Germany, Cat# 83.1832). .. For SDS-PAGE, 12 μl protein sample was mixed with 3 μl fourfold sample buffer and heated at 95°C for 5 min. SDS-PAGE and western blot was performed as described in section “2.2 Detection and verification of the Fc recombinants.” For protein detection the membranes were incubated at 4°C over night on a rocker with following primary antibodies: anti-phospho extracellular signal-regulated kinases (pErk; 1:1000; rabbit; Cell Signaling Technology; Cat# 9101; RRID:AB_331646 ), anti-total Erk (tErk; 1:1000; mouse; Santa Cruz Biotechnology; Cat# sc-271269; RRID:AB_10611091 ), anti-phospho Akt (pAkt; 1:2000; rabbit; IgG; Cell Signaling Technology; Cat# 4060; RRID:AB_2315049 ), anti-total Akt (tAkt; 1:1000; rabbit; IgG; Cell Signaling Technology; Cat# 4691; RRID:AB_915783 ), anti-phospho focal adhesion kinase (pFAK; 1:1000; rabbit; Cell Signaling Technology; Cat# 3283; RRID:AB_2173659 ), anti-total FAK (tFAK; 1:1000; rabbit; Cell Signaling Technology; Cat# 3285; RRID:AB_2269034 ), anti-notch (1:1000; rabbit; IgG; Cell Signaling Technology; Cat# 3608; RRID:AB_2153354 ), anti-cleaved notch (1:1000; rabbit; IgG; Cell Signaling Technology; Cat# 4147; RRID:AB_2153348 ), anti-Sam68 (Src associated in mitosis, of 68 kDa; 1:500; mouse; IgG; Santa Cruz Biotechnology; Cat# sc-1238; RRID:AB_627858 ) and anti-α-tubulin (1:10000; mouse; IgG; Sigma-Aldrich; Cat# T9026; RRID:AB_477593 ; see ) diluted in 5% (w/v) milk powder in 1xTBST. .. The membranes were washed thrice with 1x TBST and incubated at room temperature for 1 h on a rocker with following HRP-coupled secondary antibodies: anti-mouse [1:5000 (1:10000 for α-tubulin); goat; IgG; Jackson ImmunoResearch Labs; Cat# 115-035-068; RRID:AB_2338505 ] and anti-rabbit (1:5000; goat; IgG; Jackson ImmunoResearch Labs; Cat# 111-035-144; RRID:AB_2307391 ; see ) diluted in 5% (w/v) milk powder in 1xTBST.

    Western Blot:

    Article Title: Substrate-bound and soluble domains of tenascin-C regulate differentiation, proliferation and migration of neural stem and progenitor cells
    Article Snippet: Cells were detached from the dish using a cell scraper (Sarstedt, Nümbrecht, Germany, Cat# 83.1832). .. For SDS-PAGE, 12 μl protein sample was mixed with 3 μl fourfold sample buffer and heated at 95°C for 5 min. SDS-PAGE and western blot was performed as described in section “2.2 Detection and verification of the Fc recombinants.” For protein detection the membranes were incubated at 4°C over night on a rocker with following primary antibodies: anti-phospho extracellular signal-regulated kinases (pErk; 1:1000; rabbit; Cell Signaling Technology; Cat# 9101; RRID:AB_331646 ), anti-total Erk (tErk; 1:1000; mouse; Santa Cruz Biotechnology; Cat# sc-271269; RRID:AB_10611091 ), anti-phospho Akt (pAkt; 1:2000; rabbit; IgG; Cell Signaling Technology; Cat# 4060; RRID:AB_2315049 ), anti-total Akt (tAkt; 1:1000; rabbit; IgG; Cell Signaling Technology; Cat# 4691; RRID:AB_915783 ), anti-phospho focal adhesion kinase (pFAK; 1:1000; rabbit; Cell Signaling Technology; Cat# 3283; RRID:AB_2173659 ), anti-total FAK (tFAK; 1:1000; rabbit; Cell Signaling Technology; Cat# 3285; RRID:AB_2269034 ), anti-notch (1:1000; rabbit; IgG; Cell Signaling Technology; Cat# 3608; RRID:AB_2153354 ), anti-cleaved notch (1:1000; rabbit; IgG; Cell Signaling Technology; Cat# 4147; RRID:AB_2153348 ), anti-Sam68 (Src associated in mitosis, of 68 kDa; 1:500; mouse; IgG; Santa Cruz Biotechnology; Cat# sc-1238; RRID:AB_627858 ) and anti-α-tubulin (1:10000; mouse; IgG; Sigma-Aldrich; Cat# T9026; RRID:AB_477593 ; see ) diluted in 5% (w/v) milk powder in 1xTBST. .. The membranes were washed thrice with 1x TBST and incubated at room temperature for 1 h on a rocker with following HRP-coupled secondary antibodies: anti-mouse [1:5000 (1:10000 for α-tubulin); goat; IgG; Jackson ImmunoResearch Labs; Cat# 115-035-068; RRID:AB_2338505 ] and anti-rabbit (1:5000; goat; IgG; Jackson ImmunoResearch Labs; Cat# 111-035-144; RRID:AB_2307391 ; see ) diluted in 5% (w/v) milk powder in 1xTBST.



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